Synonyms: CNTF,HCNTF,Ciliary neurotrophic factor

Accession: P26441

Description：CNTF protein is a neurotrophic factor that promotes neuronal survival and differentiation. It is involved in the regulation of various cellular processes, such as inflammation, metabolism, and immune response. CNTF has potential therapeutic applications for the treatment of neurodegenerative diseases.

Source: HEK293 cells-derived human CNTF protein, Met1-Met200, with N-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human CNTF consists of 206 amino acids and The protein has a predicted MW of 24.01 kDa. And it migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/mL, corresponding to a specific activity of > 6.6 × 10³ IU/mg. Fully biologically active when compared to standard. 

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: