Synonyms: IL4, BCGF1, BSF1

Accession: P05112

Description：IL-4 is a protein that regulates the immune response by promoting the growth and differentiation of B cells. It also plays a role in the development of allergic reactions and asthma. IL-4 is produced by a variety of cells, including T cells and mast cells.

Source: HEK293 cells-derived human IL-4 protein, His25-Ser153, with C-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human IL-4 consists of 136 amino acids and The protein has a predicted MW of 16.05 kDa. And it migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of >1 × 10⁷ IU/mg. Fully biologically active when compared to standard. 

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: