Synonyms: KITLG, FPH2, KL-1, Kitl, MGF, SF, SHEP7, KL

Accession: P21860

Description：SCF, also known as stem cell factor or kit ligand, is a cytokine that plays a critical role in the survival, proliferation, and differentiation of hematopoietic stem cells and early progenitor cells. SCF is primarily produced by bone marrow stromal cells and functions by binding to the c-kit receptor on the surface of hematopoietic cells. Dysregulation of SCF signaling has been implicated in various types of cancer and hematological disorders.

Source: HEK293 cells-derived human SCF protein, Glu26-Ala189, with N-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human SCF consists of 171 amino acids and The protein has a predicted MW of 20.41 kDa. And it migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using MO7e human megakaryocytic leukemic cells is 0.77-1.17 ng/mL, corresponding to a specific activity of >8.5 × 10⁵ IU/mg. Fully biologically active when compared to standard.

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: