Synonyms: FGF-10, Fibroblast growth factor 10, Keratinocyte growth factor 2
Accession: O15520
Description:Human FGF-10 protein, also known as Fibroblast Growth Factor-10, is a growth factor that belongs to the fibroblast growth factor family. It plays a crucial role in the development and repair of various tissues, particularly in the respiratory and limb development.
Source: E.coli-derived human FGF-10 protein, Gln38-Ser208, with C-terminal His tag.
Purity: ≥90% as determined by SDS-PAGE.
Endotoxin: < 1 EU per 1μg of the protein by the LAL method.
Predicted N terminal: Met.
Molecular Mass: The recombinant human FGF-10 consists of 178 amino acids and has a calculated molecular mass of 20.4 kDa.
Formulation: Supplied as sterile PBS, pH 7.4.
SDS-PAGE:
Human FGF-10, his tag immobilized on CM5 chip can bind human FGFR2 alpha(IIIb) with an affinity constant of 9.69 x 10-9 M as determined in a SPR assay (Biacore 8K).
Handling: Centrifuge vial prior to opening.
Do not vortex after reconstitution.
Avoid repeated freeze-thaw cycles.
Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.
Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.
Storage/Stability:
Product Form | Temperature | Storage Time |
Lyophilized | -20℃ to -80℃. | 2 years |
Lyophilized | 4°C | 6 months |
Lyophilized | Room Temperature | 1 month |
Reconstituted | 2°C to 8°C | 1 week |
Extended Storage | -20℃ to -80℃. | 1 year |