Synonyms: IL33, DV27, C9ORF26, IL1F11, NFHEV, DKFZp586H0523, DVS27, NFEHEV, RP11-575C20.2

Accession: O95760-1

Description：IL-33, also known as interleukin-33, is a protein that belongs to the IL-1 superfamily. It is primarily produced by epithelial and endothelial cells and acts as an important cytokine involved in immune response and inflammation. IL-33 functions as an alarmin, which means it is released upon tissue damage and initiates an inflammatory response to recruit immune cells to the site of injury.

Source: E.coli-derived human IL-33 protein, His109-Thr 270, with C-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human IL-33 consists of 169 amino acids and The protein has a predicted MW of 19.4 kDa.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

Human IL-1RL1 immobilized on CM5 chip can bind human IL-33 with an affinity constant of 8.14×10^-11 M as determined in a SPR assay (Biacore 8K).

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: