Synonyms: BDNF, ANON2, BULN2, Abrineurin

Accession: P23560-1

Description：BDNF, short for Brain-Derived Neurotrophic Factor, is a protein that a crucial role in the growth, development, and survival of neurons in the brain. It is primarily produced in the hippocampus, cortex, and basal forebrain regions of the brain. BDNF is a vital protein in the brain that supports neuronal growth, survival, and synaptic plasticity. Its role in cognition, mood regulation, and neuroprotection make it a subject of significant scientific investigation.

Source: HEK293 cells-derived human BDNF protein, Ala19-Arg247, with N-terminal Flag tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human BDNF consists of 238 amino acids and The protein has a predicted MW of 26.75 kDa. And it migrates as 20-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

Human TRKB immobilized on CM5 chip can bind human BDNF, Flag tag, with an affinity constant of 3.86 × 10^-10 M as determined in a SPR assay (Biacore 8K).

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: