Synonyms: EGF, URG, HOMG4
Accession: P01133
Description:Human epidermal growth factor (EGF) protein is a naturally occurring protein that plays a vital role in cell growth and development. It is produced and released by various cells in the body, including the skin, salivary glands, and kidneys. human EGF protein is a vital protein in the body that plays a crucial role in cell growth, tissue regeneration, and overall health. Its inclusion in various skincare products has made it a popular ingredient in the pursuit of healthier and more youthful-looking skin.
Source: E.coli-derived human EGF protein, Asn971-Arg1023, with C-terminal His tag.
Purity: ≥90% as determined by SDS-PAGE.
Endotoxin: < 1 EU per 1μg of the protein by the LAL method.
Human EGF, His tag, immobilized on CM5 chip can bind EGFR with an affinity constant of 7.14 x 10-6 M as determined in a SPR assay (Biacore 8K).
Handling: Centrifuge vial prior to opening.
Do not vortex after reconstitution.
Avoid repeated freeze-thaw cycles.
Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.
Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.
Storage/Stability:
Product Form | Temperature | Storage Time |
Lyophilized | -20℃ to -80℃. | 2 years |
Lyophilized | 4°C | 6 months |
Lyophilized | Room Temperature | 1 month |
Reconstituted | 2°C to 8°C | 1 week |
Extended Storage | -20℃ to -80℃. | 1 year |
Predicted N terminal: Met.
Molecular Mass: The recombinant human EGF consists of 60 amino acids and has a calculated molecular mass of 7.3 kDa.
Formulation: Supplied as sterile PBS, pH 7.4.