Synonyms: Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF

Accession: P01375

Description：Human TNF-alpha protein, also known as tumor necrosis factor alpha, is a cytokine that plays a crucial role in the inflammatory response. It is produced by various immune cells such as macrophages and T-cells and is involved in the regulation of immune cell proliferation, differentiation, and apoptosis. TNF-alpha is also known to induce fever, stimulate prostaglandin and nitric oxide production, and increase vascular permeability. Dysregulation of TNF-alpha has been implicated in various diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis. Therapeutic strategies targeting TNF-alpha have been developed and successfully used in the treatment of these diseases.

Source: E.coli-derived human TNF-α/TNFSF2 protein, Val77-Leu233, with C-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human TNF-α/TNFSF2 consists of 164 amino acids and has a calculated molecular mass of 18.5 kDa.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is 0.095 to 0.106 ng/mL, corresponding to a specific activity of > 9.4 × 10⁶ IU/mg.

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: