Synonyms: LPF;TCGF-2;HCGF-21

Accession: P13232

Description：Interleukin 7 (IL-7) is a cytokine involved in the regulation of immune cell development and survival, including T and B lymphocytes. It is produced by stromal cells, such as fibroblasts and bone marrow stromal cells. Dysregulation of IL-7 signaling has been implicated in autoimmune diseases and cancer.

Source: HEK293 cells-derived human IL-7 protein, Asp26-His177, with N-terminal his tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human IL-7 consists of 159 amino acids and has a calculated molecular mass of 17.34 kDa. And it migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE:

The ED50 as determined by a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) is less than 1 ng/mL, corresponding to a specific activity of >1 x 10⁶ IU/mg. Fully biologically active when compared to standard.

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: