Synonyms: MVCD1, VAS, vascular endothelial growth factor A, VEGF, VEGFA, VPF

Accession: P15692-4

Description：Human VEGF 165 protein, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF. Human VEGF 165 protein is a crucial regulator of angiogenesis, with implications in various physiological and pathological processes. Its study contributes to our understanding of blood vessel development, tissue repair, and the development of therapeutic strategies for diseases such as cancer.

Source: HEK293 cells-derived human VEGF165 protein, Ala27-Arg191, with C-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human VEGF165 consists of 172 amino acids and The protein has a predicted MW of 20.25 kDa. And it migrates as 20-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

Human VEGFR1 immobilized on CM5 chip can bind human VEGF 165, his tag with an affinity constant of 1.26 x 10-¹⁰ M as determined in a SPR assay (Biacore 8K).

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: