Synonyms: FGF19;Fibroblast growth factor 19

Accession: O95750

Description：FGF-19 is a member of the fibroblast growth factor (FGF) family. After being secreted from the intestine, FGF19 can enter the liver with the circulation and bind to FGFR4 in the liver to act. It has a hormone-like effect and plays an important role in metabolic regulation, such as regulating bile acid metabolism, regulating gallbladder filling, and improving blood glucose. Notably, aberrant expression of FGF19/FGFR4 promotes HCC development and metastasis.

Source: HEK293 cells-derived human FGF-19 protein, Leu 25-Lys 216, with N-terminal Flag tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human FGF-19 consists of 200 amino acids and The protein has a predicted MW of 23.36 kDa. And it migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/mL

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Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: