Synonyms: IL3, MCGF, MGC79398, MGC79399, MULTI-CSF, Interleukin-3

Accession: P08700-1

Description：Human IL-3 protein, also known as Interleukin-3, is a cytokine that is produced by activated T cells and mast cells. It plays a crucial role in regulating the growth, differentiation, and function of various cell types in the immune system. human IL-3 protein is an important cytokine that regulates the growth, differentiation, and function of immune cells. Its study contributes to our understanding of immune system regulation and its potential applications in immunotherapy and disease treatment.

Source: HEK293 cells-derived human IL-3 protein, Ala20-Phe152, with N-terminal Flag tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human IL-3 consists of 142 amino acids and The protein has a predicted MW of 16 kDa. And it migrates as 20-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg. Fully biologically active when compared to standard.

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: