Synonyms: IGF-I, IGF1A, somatomedin C, MGF

Accession: P05019-1

Description：IGF-1, also known as insulin-like growth factor 1, is a protein that plays a crucial role in growth and development. It is produced mainly by the liver and other tissues in response to growth hormone secretion. IGF-1 is a vital protein in human physiology, contributing to growth, development, and maintenance of various tissues and metabolic processes.

Source: E.coli-derived human IGF-1 protein, Gly49-Ala118, with C-terminal Flag tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human IGF-1 consists of 78 amino acids and has a calculated molecular mass of 8.6 kDa.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. Fully biologically active when compared to standard. 

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: