Synonyms: CD154 antigen, CD154, CD40 antigen ligand, CD40 Ligand, gp39, HIGM1, T-B cell-activating molecule, T-BAM, TNFSF5, TNFSF5IMD3, TRAP

Accession: P29965

Description：Human sCD40 Ligand protein, also known as soluble CD40 Ligand protein, is a protein produced by activated T cells. It plays a crucial role in the immune response by binding to its receptor, CD40, which is expressed on the surface of B cells, dendritic cells, and other immune cells.

Source: HEK293 cells-derived human sCD40 Ligand protein, Met113-Leu261, with N-terminal His tag.

Purity: ≥90% as determined by SDS-PAGE.

Endotoxin: < 1 EU per 1μg of the protein by the LAL method.

Predicted N terminal: Met.

Molecular Mass: The recombinant human sCD40 Ligand consists of 156 amino acids and has a calculated molecular mass of 17.36 kDa. And it migrates as 17-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Formulation: Supplied as sterile PBS, pH 7.4.

SDS-PAGE: 

Human CD40 immobilized on CM5 chip can bind human sCD40 Ligand, his tag with an affinity constant of 2.31×10^-9 M as determined in a SPR assay (Biacore 8K).                                

Handling: Centrifuge vial prior to opening.

Do not vortex after reconstitution.

Avoid repeated freeze-thaw cycles.

Reconstitution: Initially reconstitute in water to 0.1-1.0 mg/ml. Store at 2℃ to 8℃ for up to 1 week or prepare for extended storage.

Extended Storage: After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20℃ to 80℃.

Storage/Stability: